Molecular biology has transitioned to a new era where precise genetic manipulation plays a critical role in research, medicine, and biotechnology. Among the vital tools in this field are cloning ...

The basic technology of genetic engineering is artificial cutting, splicing, and combination of genes. A gene is a DNA molecule with a certain function. To accurately cut out the DNA linear molecular ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

The endoglycosidase heparanase is an important in the degradation of the extracellular matrix by invading cells, notably metastatic tumor cells and migrating leukocytes. Here we report the cDNA ...

In tobacco (Nicotiana tabacum L. cv Samsun NN), three distinct enzymes account for ortho-diphenol-O-methyltransferase (OMT) activity. OMT I is the major enzyme of healthy leaves, whereas enzymes OMT ...

In order to investigate a possible association between soybean malate synthase (MS; L-malate glyoxylate-lyase, CoA-acetylating, EC 4.1.3.2) and glyoxysomal malate dehydrogenase (gMDH; (S)-malate: NAD+ ...