“Where PCR is really going,” says Olivier Harismendy at the University of California, San Diego, “is parallelization and miniaturization.” Indeed, researchers are making use of a wide variety of ...

The polymerase chain reaction (PCR) is a molecular method that is employed for in vitro amplification of a specific region in a strand of deoxyribonucleic acid (DNA). Multiplex PCR involves the ...

The changing technology landscape will affect the BioWatch program. This chapter comments on additional assay performance considerations that arise from the use of multiplexed real-time polymerase ...

The modular multiplex RT-PCR method simultaneously detects Shiga Toxin E. coli (STEC), Salmonella spp. and Listeria monocytogenes using a single, overnight enrichment (24 hours). Ready-to-use PCR ...

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High-throughput discovery technologies deliver an astounding quantity of data but often the greater challenge is to identify real targets from the vast body of potential hits. Typically, this task ...

Molecular analysis using PCR techniques demands both precision and versatility, integrating methods that offer unique yet complementary strengths. However, researchers can face challenges balancing ...

Multiplex PCR is a difficult technique that allows amplification of two or more products in parallel in a single reaction tube. This technology was first described by Chamberlain et al., in 1988, and ...

Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics. As a sensitive and precise method for quantitating specific nucleic acid targets, qPCR is ...

There are many different technologies available to conduct multiplex PCR. However, each comes with its own sets of pros and cons. This infographic compares three pillars of PCR, quantitative PCR, ...

A team of UK-based scientists has recently developed an allele-specific probe polymerase chain reaction method that can accurately detect different variants/lineages of severe acute respiratory ...